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The following question has a paragraph from which the last sentence has been deleted. From the given options, choose the one that completes the paragraph in the most appropriate way.

Research on animals is performed to gain more knowledge about diseases and how to cure them, and to evaluate drugs for toxicity before testing them on humans. In fact, animal studies have played a vital role in almost every major medical advance.Gaining useful knowledge from animal research requires robust experimental findings: different scientists should be able to reproduce them in different locations. _________________
OPTIONS
 
 This requires a thorough understanding of each animal species and its biology.
 However, evidence is gradually accumulating that the majority of research animals are mentally stressed by their living conditions.
 Between 50 and 100 million animals, from flies to monkeys, are euthanized for research each year worldwide.
 Many scientists, however, are unaware that their animal facilities can affect research outcomes and compromise their data.
 However, rigorous standardization of the environment increases the risk of obtaining results that, being specific to a narrow set of conditions, cannot be compared with other researchers’ results.

 
 
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